MACROMOLECULAR ENGINEERING for BIOMEDICAL APPLICATIONS

AIM

The course will cover some polymerization and bioconjugate techniques that commonly used for biomedical application. Synthesis approach and bioconjugation techniques will be given as basic information by considering interdisciplinary student profile. The course will initially focus on basic polymerization techniques and characterization methods for these macromolecues. Later, it will cover some bioconjugation techniques based on their application such as creating specific functionality, degradable reagents, fluorescent probes, and PEGylation. The class will rely on interactive discussion after giving related synthetic approach which will be supported by multiple examples from relevant literature.

CONTENT

This course contains; Introduction to Polymer Chemistry,Polymerization Techniques,Polymerization Techniques,Characterization of Bioconjugated Macromolecules,Bioconjugation Strategies and Design,Functional Targets for Bioconjugation and Creating Specific Functionality,PEGylation,Homo and Heterobifunctional Crosslinkers,Cleavable Reagents,Fluorescent Probes,Isotop Labeling Techniques,Micro and Nanoparticles,Liposome Conjugates,Antibody Modification and Conjugation.

1. 1

   Recognize macromolecular design/synthesis and common bioconjugation techniques.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

2. 2

   Distinguish the structure-properties relationships based on synthesis techniques.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

3. 3

   Understands the techniques required for specific applications.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Experimental Technique, Micro Teaching Technique, Brainstorming Technique, Lecture Method · Assessed by: Project Task

4. 4

   Suggest the type of chemical modification for specific application.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

5. 5

   Interpret the function of conjugates and functional groups.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

6. 6

   Interpret the type of bioconjugation and the linker that needs to be used.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

7. 7

   Identify the modification that has been done and the response that will be generated by the system.

   Taught by: Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

8. 8

   Analyse the requirements of bioconjugated macromolecules.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

9. 9

   Compare various technologies utilized for macromolecular engineering.

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

10. 10

    Summarize the basic constructs used for bioconjugation.

    Taught by: Self Study Method, Question - Answer Technique, Micro Teaching Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

11. 11

    Recognize the frequently used component to obtain certain properties on a bioconjugate.

    Taught by: Self Study Method, Question - Answer Technique, Micro Teaching Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

12. 12

    Design basic polymerization system and bioconjugates.

    Taught by: Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

WEEKLY PLAN

1. WEEK 1

   Introduction to Polymer Chemistry

   Preparation: Week 1 Presentation PDF

2. WEEK 2

   Polymerization Techniques

   Preparation: Week 2 Presentation PDF

3. WEEK 3

   Polymerization Techniques

   Preparation: Week 3 Presentation PDF

4. WEEK 4

   Characterization of Bioconjugated Macromolecules

   Preparation: Week 4 Presentation PDF

5. WEEK 5

   Bioconjugation Strategies and Design

   Preparation: Week 5 Presentation PDF

6. WEEK 6

   Functional Targets for Bioconjugation and Creating Specific Functionality

   Preparation: Week 6 Presentation PDF

7. WEEK 7

   PEGylation

   Preparation: Week 7 Presentation PDF

8. WEEK 8

   Homo and Heterobifunctional Crosslinkers

   Preparation: Week 8 Presentation PDF

9. WEEK 9

   Cleavable Reagents

   Preparation: Week 9 Presentation PDF

10. WEEK 10

    Fluorescent Probes

    Preparation: Week 10 Presentation PDF

11. WEEK 11

    Isotop Labeling Techniques

    Preparation: Week 11 Presentation PDF

12. WEEK 12

    Micro and Nanoparticles

    Preparation: Week 12 Presentation PDF

13. WEEK 13

    Liposome Conjugates

    Preparation: Week 13 Presentation PDF

14. WEEK 14

    Antibody Modification and Conjugation

    Preparation: Week 14 Presentation PDF

ASSESSMENT

• Rate of Midterm Exam to Success50%
• Rate of Final Exam to Success50%

WORKLOAD

READING

• Lecturer will provide course materials as “ppt” files
• Bioconjugate Techniques, Greg T. Hermanson, 3rd edition

TEACHING STAFF

• Prof.Dr. Yasemin YÜKSEL DURMAZCOORDINATOR
• Prof.Dr. Yasemin YÜKSEL DURMAZ