MACROMOLECULAR ENGINEERING for BIOMEDICAL APPLICATIONS

AIM

The course will cover some polymerization and bioconjugate techniques that commonly used for biomedical application. Synthesis approach and bioconjugation techniques will be given as basic information by considering interdisciplinary student profile.

CONTENT

This course contains; Introduction to Polymer Chemistry,Polymerization Techniques,Controlled Polymerization Techniques,Characterization of Bioconjugated Macromolecules,Bioconjugation Strategies and Design,Functional Targets for Bioconjugation and Creating Specific Functionality,PEGylation,Homo and Heterobifunctional Crosslinkers,Cleavable Reagents,Fluorescent Probes,Isotop Labeling Techniques,Micro and Nanoparticles,Liposome Conjugates,Antibody Modification and Conjugation.

1. 1

   Recognize macromolecular design/synthesis and common bioconjugation techniques

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

2. 2

   Distinguish the structure-properties relationships based on synthesis techniques

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

3. 3

   Distinguish the need of using special techniques for certain applications

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

4. 4

   Suggest the type of chemical modification for specific application

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

5. 5

   Interpret the function of conjugates and functional groups

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

6. 6

   Interpret the type of bioconjugation and the linker that needs to be used

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

7. 7

   Identify the modification that has been done and the response that will be generated by the system

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

8. 8

   Analyse the requirements of bioconjugated macromolecules

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

9. 9

   Compare various technologies utilized for macromolecular engineering

   Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

10. 10

    Summarize the basic constructs used for bioconjugation

    Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

11. 11

    Recognize the frequently used component to obtain certain properties on a bioconjugate

    Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

12. 12

    Design basic polymerization system and bioconjugates

    Taught by: Discussion Method, Self Study Method, Question - Answer Technique, Brainstorming Technique, Lecture Method · Assessed by: Traditional Written Exam, Project Task

WEEKLY PLAN

1. WEEK 1

   Introduction to Polymer Chemistry

   Preparation: Going through course materials

2. WEEK 2

   Polymerization Techniques

   Preparation: Going through course materials

3. WEEK 3

   Controlled Polymerization Techniques

   Preparation: Going through course materials

4. WEEK 4

   Characterization of Bioconjugated Macromolecules

   Preparation: Going through course materials

5. WEEK 5

   Bioconjugation Strategies and Design

   Preparation: Going through course materials and reeding recommended articles

6. WEEK 6

   Functional Targets for Bioconjugation and Creating Specific Functionality

   Preparation: Going through course materials

7. WEEK 7

   PEGylation

   Preparation: Going through course materials and reeding recommended articles

8. WEEK 8

   Homo and Heterobifunctional Crosslinkers

   Preparation: Going through course materials

9. WEEK 9

   Cleavable Reagents

   Preparation: Going through course materials

10. WEEK 10

    Fluorescent Probes

    Preparation: Going through course materials and reeding recommended articles

11. WEEK 11

    Isotop Labeling Techniques

    Preparation: Going through course materials and reeding recommended articles

12. WEEK 12

    Micro and Nanoparticles

    Preparation: Going through course materials and reeding recommended articles

13. WEEK 13

    Liposome Conjugates

    Preparation: Going through course materials and reeding recommended articles

14. WEEK 14

    Antibody Modification and Conjugation

    Preparation: Going through course materials and reeding recommended articles

ASSESSMENT

• Rate of Midterm Exam to Success50%
• Rate of Final Exam to Success50%

WORKLOAD

READING

• Course materials
• Bioconjugation Techniques, Greg T. Hermanson, 3rd edition

TEACHING STAFF

• Prof.Dr. Yasemin YÜKSEL DURMAZCOORDINATOR
• Prof.Dr. Yasemin YÜKSEL DURMAZ